Detection of mouse IgG specific to IgE, IgE(PNG), and Fel d 1 and
their subclasses after immunization with IgE-ICs
Plates were coated with mouse IgE F127, IgE(PNG), or Fel d 1. Serial
dilution of sera (1:25, then serially 1:3) was added. HRP-labeled goat
anti-mouse IgG (The Jackson Laboratory) antibodies were used to detect
IgG (1:2’000). To detect IgG subclasses, HRP labeled rat anti-mouse IgG1
(PD Pharmingen), goat anti-mouse IgG2a (Bio-Rad), goat anti-mouse IgG2b
(Invitrogen, Carlsbad, CA, USA), and goat anti-mouse IgG3
(SouthernBiotech, Birmingham, AL, USA) were used in a 1:2’000 dilution.