Detection of mouse IgG specific to IgE, IgE(PNG), and Fel d 1 and their subclasses after immunization with IgE-ICs
Plates were coated with mouse IgE F127, IgE(PNG), or Fel d 1. Serial dilution of sera (1:25, then serially 1:3) was added. HRP-labeled goat anti-mouse IgG (The Jackson Laboratory) antibodies were used to detect IgG (1:2’000). To detect IgG subclasses, HRP labeled rat anti-mouse IgG1 (PD Pharmingen), goat anti-mouse IgG2a (Bio-Rad), goat anti-mouse IgG2b (Invitrogen, Carlsbad, CA, USA), and goat anti-mouse IgG3 (SouthernBiotech, Birmingham, AL, USA) were used in a 1:2’000 dilution.