Assay for assessing IgE and IgE-IgG complexes clearance in serum
Anti-mouse IgE STAR110 (Bio-Rad) was coated. Then, serial dilution of sera (1:25, then 1:3 serially) was added. Goat anti-mouse IgE-HRP STAR110P (Bio-Rad) was used to detect mouse IgE (1:2’000), and HRP-labeled anti-mouse IgG was used to detect IgE-IgG complexes (1:2’000).