ELISA Assays
96-well Nunc MaxisorpTM ELISA plates (Thermo Fisher) were coated with 2µg/ml antibody in PBS at 4°C overnight. After blocking with PBS/0.15% Casein solution for 2h at room temperature, plates were washed 5x with PBS. Incubation of antibodies or sera was performed for 1h at RT. Afterward, plates were washed 5x with PBS/0.05% Tween. ELISAs were developed with TMB (3,30,5,50-tetramethylbenzidine) and H2O2 and stopped with 1mol/L sulfuric acid after 10 minutes. OD was measured at 450 nm.