Passive Immunization and Challenge
Mice were injected with 25µg anti-IgE IgG, 25µg anti-IgE(PNG) IgG, or 25µg control IgG (Purified mouse IgG1, k, MOPC-31C, BD Bioscience) in 100µl PBS i.v. 24 hours later, mice were sensitized with 5µg IgE F127 or 5µg anti-Ara h2 IgE in 100µl PBS. The next day, baseline body temperature was measured by MiniTemp rectal probe for mice (Vetronic Services Ltd, Abbotskerswell, UK). IgE sensitized mice were challenged by intravenous injection with 5µg Fel d 1 or 20µg roasted peanut extract (Ara R), and rectal temperature was measured at 10min-intervals for 1h.