Assessment of anti-IgE IgG purification
Plates were coated with IgE, IgE(PNG), or Fel d 1. The eluate and flow-through of the protein G and Fel d 1-column were tested starting with 2µg/ml and further serially diluted 1:3. HRP-labeled goat anti-mouse IgG (The Jackson Laboratory) antibodies were used for detection of IgG (1:2’000).