Fecal anti-IgE IgA detection
Fecal pellets were collected before and after immunizing mice three times with IgE-Fel d 1 or IgE(PNG)-Fel d 1 and snap-frozen in liquid nitrogen and stored at -80°C until analysis. The pellets were resuspended in PBS in a 10mg per 100ml dilution by vortexing. Dissolved samples were centrifuged at 10’000g, and the supernatant was used for IgA analysis. ELISA plates were coated with IgE F127. HRP-labeled goat anti-mouse IgA STAR137P (Bio-Rad) was used in a 1:6’000 dilution to detect IgE-specific IgA.