RNA-Seq
PF Th17 cell subpopulations were sorted from 5 patients and pooled for RNA-Seq. Cellular RNA was extracted using the Arcturus PicoPure RNA Isolation Kit (ThermoFisher). The quality and quantities of RNA samples were measured with the QIAxcel RNA QC Kit v2.0 (Qiagen) following the manufacturer’s instructions. One microgram RNA at the concentration > 200 ng/µl was subjected to library construction, sequencing, reads quality check, alignment, clustering, expression assessment, Gene Ontology (GO) enrichment analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis by the Wuhan SeqHealth Co., Ltd. The CollibriTM Stranded RNA Library Prep Kit for Illumina (ThermoFisher), HiSeq 2000 Sequencing System (Illumina), TopHat v2.1.1, HTSeq v0.12.4, DEGseq2, and Hypergeometric test were used for the above procedures, respectively.