Figure 4. Functions of PF Th17 subsets. (A & B) GM-CSF expression in IL-17RE+CD27- subset, IL-17RE+CD27+ subset, and IL-17RE-CD27- subset. Representative flow cytometry plots are shown in (A), and statistics of the frequencies of GM-CSF+ cells are shown in (B). (C)Relative mRNA levels of IL22 in the three subsets. N=5 per group. Kruskal-Wallis test. (D) Flow cytometry plots showing cell cycle measured by staining with Hoechst 33342 and Pyronin Y. *:p <0.05; **: p <0.01.