2.3 | Sequencing and phylogenetic analysis
VP1 sequences were generated using previously described methods (Knowles et al., 2016). Complete VP1 nucleotide sequences were aligned using BioEdit 7.0.5.3 (Hall, 1999) and Clustal W 1.83 (Thompson et al., 1994). Optimal nucleotide substitution models were computed for each serotype using MEGA 7 (Kumar et al., 2016). The maximum likelihood algorithm was used to construct phylogenetic trees employing using MEGA 7. One thousand bootstrap pseudo-replicates were used to assess branching confidence.