Materials
Alternaria alternata filtrate was purchased from Stallergenes Greer Laboratories (Lenoir, NC). PAR2 agonists [2-aminothiazole-LIGR-NH2(2at-LIGRL-NH2) and 2-furoyl-LIGRLO-NH2(2f-LIGRLO-NH2)], and the PAR2 antagonist C391 were manufactured in our laboratory as described (Boitano et al., 2011; Boitano et al., 2015; Flynn et al., 2011; Hoffman et al., 2012). Unless listed below, all other chemicals/components were of Molecular Biology or higher grades and purchased from Fisher Scientific (Pittsburgh, PA), ThermoFisher Scientific (Watham, MA), Sigma-Aldrich (Burlington, MA), or VWR (West Chester, PA).