2.12 Data Analysis
P was determined by calculating the proportion of viral RNA-positive
mosquito bodies for the combined total number of mosquito RNA samples.
Dissemination was determined by calculating the proportion of legs/wings
RNA samples with detectable RVFV RNA against the total number of
mosquitoes exposed. Transmission was determined by calculating the
proportion of saliva RNA samples that were RVFV-RNA positive against the
total number of mosquitoes exposed. Percent of saliva expectorants
containing infectious virus were also calculated by determining the
proportion of saliva samples producing detectable CPE by plaque assay
among the total number of individuals tested. The percentage of
RVFV-infected mosquitoes after feeding on inoculated goats were
determined by calculating plaque positive mosquito bodies per total
number of mosquitoes assayed. RVFV growth curve titers were analyzed by
calculating the highest dilution containing countable plaques and
multiplying that by the dilution factor to obtain log10PFU/ml.
All graphing and statistical tests were performed in Prism Graphpad
(version 8, https://www.graphpad.com/). χ2 contingency
tests were used to calculate dissemination and transmission rates. Two
way ANOVA (analysis of variance) with Geisser-Greenhouse correction was
used to determine differences in viral growth kinetics. One way ANOVA
was used to determine differences in bloodmeal titers.