Fig.1 Isolation and identification of PDCoV
(a) The samples were tested by RT-PCR to determine that the samples were
infected with PDCoV and no other common virus were present. (b)
Isolation and passage of PDCoV in LLC-PK cells. Magnification: 200×. (c)
Indirect immunofluorescence assay on LLC-PK cells infected with PDCoV.
(d) Indirect immunofluorescence assay on the intestine of PDCoV-infected
pigs. The primary antibody is a monoclonal antibody against N protein.