FIGURE 3. Scheme for optimization of in silico mate-pair method (map method). Mate pairs generated from the first (1st) reference were mapped to the second (2nd) reference. Only mapped mate pairs that satisfied the insert size of “0.8 L n ≤ L n’ ≤1.2 L n” and that were also in the same direction were reserved for the following scaffolding process during genome assembly.