3 Results and Discussion
The four most stable conformations of probe C23 were found as shown in
Figure1. The difference among the four conformations were clearly
depicted in the front and side view of the molecular structure of the
probes due to the rotational bonds. The transfer among the different
conformations provided the non-radiation energy loss when the probe C23
recovered back to the ground state from excited states, led to no
fluorescence generation with the probe C23 itself. When the probe was
incorporated into a hydrophobic cavity of HSA, the bonding interaction
between the probe and HSA resulted in the inhibition of non-irradiative
energy loss pathways and an increase in fluorescence within the red
channel.