4. Conclusions
A high-quality chromosome-level genome assembly of the galling aphidS. chinensis was established in this study. Phylogenetic analysis
indicated that S. chinensis diverged from E. lanigerum at
approximately 57 million years ago (MYA). Transcriptome analysis showed
that 35 genes that encoded salivary gland proteins were highly expressed
in the gall forming fundatrix stage. Some of these salivary proteins
might be involved in gall formation. Our results will benefit future
research to study the molecular mechanisms underlying the unique biology
associated with galling aphids, their gall induction ability, and
molecular interactions between insects and plants.
Data accessibility
statement
All
data mentioned in this paper have been deposited in the National Center
for Biotechnology Information with the BioProject accession number
PRJNA700780 (genomic sequencing) and PRJNA702264 (transcriptome
sequencing). The final DNA sequence assembly has been deposited at
DDBJ/ENA/GenBank under the accession JAFHKX000000000.
The genome assembly and
annotation, orthogroup clustering results and salivary gland genes are
available for download from Zenodo (10.5281/zenodo.3797131).