2.9 Identification of genes potentially involved in gall
formation and host manipulation
One hundred forty-one proteins were identified from the saliva ofS. chinensis in a previous study (Yang et al., 2018). These
identified proteins were used to identify genes potentially involved in
gall formation and host manipulation. tBLASTN was used to search the
corresponding genes in the S. chinensis genome with the 141
salivary proteins as queries (E-value ≤1×10−5,
identify ≥ 50). The expression levels of salivary protein-encoding genes
were quantified in three stages based on the RNA-seq data. Up-regulated
genes in fundatrix were subject to GO and KEGG enrichment analyses using
Omicshare CloudTools with default parameters
(http://www.omicshare.com/).