Experimental Approach
Pseudotyped SARS-CoV-2 entry assay and dose-response curve analysis with
authentic SARS-CoV-2 and recombinant SARS-CoV-2 reporter virus
expressing the nanoluciferase were carried out to investigate the
effects of compounds against SARS-CoV-2 entry into host cells. Filipin
cholesterol staining, SARS-CoV-2 Spike (S)-ACE2 binding assay, and
S-mediated cell fusion assay using time-lapse imaging, flow cytometry,
and split-GFP fusion were conducted to understand the inhibitory
mechanisms.