2.14 Western blot analysis
Protein samples from the cells of split-GFP assay (incubation time was
prolonged to 72 h) were prepared by dissolving the cultures directly in
2X SDS sample buffer and analyzed by western blot analysis. The primary
antibodies used were as follows; rabbit anti-cleaved caspase-3 and
caspase-9 (Cell Signaling #9664 and #52873), and mouse anti-GAPDH
(Abcam, ab8245). We used secondary antibodies conjugated with
horseradish peroxidase (KPL, USA), and developed the blots using ECL
Western Blotting Substrate (Thermo Fisher Scientific, USA). Images of
original uncropped western blots were provided (Fig. S4).