2.2 Extraction and Isolation
The fresh aerial parts (289 g) and roots (209 g) were sliced and
extracted in an ultrasonic bath (room temperature for 2 hours with 3 L
and 2 L of 70% EtOH, respectively) five times. The extracts were
filtered and concentrated by a rotary evaporator to give 70% EtOH
extracts of the aerial parts (29.4 g, 10.20%) and roots (23.5 g,
11.24%). The eight compounds tested in this study, tangshenoside I (1),
ethylsyringin (2), syringin (3), sinapaldehyde glucoside (4), lobetyolin
(5), lancemasides A (6), B (7), and D (8), were previously isolated from
the roots of CL (Du et al., 2018).