2.3 Sequence alignment, phylogenetic analysis and molecular
identification
Sequences generated from this study were combined with sequences
obtained from GenBank. Exserohilum turcicum and Cocoicola
californica were selected as the outgroups. Sequence data was aligned
and concatenated using MAFFT v.7
(mafft.cbrc.jp/alignment/server/index/html) using the G-INS-I model and
manually inspected. The best fit partitioning schemes were determined
using PartitionFinder (Lanfear et al. 2017) and used to build the
phylogenies. Both single gene and concatenated gene sets were analyzed
using a maximum likelihood (ML) analysis. The ML phylogenies were
generated by RaxML under GTR model with gamma distributed rate
heterogeneity with 1000 bootstrap replicates. Resulting trees were
visualized with iTOL (Interactive Tree of Life) v.6
(https://itol.embl.de/) or MEGA.
Sequences generated in this study were deposited in GenBank (Table 1)
RESULTS