Figure Legend
Figure 1. Signs and symptoms of Phyllachora spp . on
grasses. P. maydis on maize at severe levels (A); with ascospores
being extruded from stroma (B) and showing characteristic tapering ends
of mature stromata (C). Phyllachora spp . on Elymus in
Michigan (D), Fall Ryegrass in Illinois \euro, and an unidentified
grass in Indiana (F). Photo credit N. Kleczewski
Figure 2 . Maximum likelihood phylogenetic tree based on
combined ITS and LSU sequence data from the stroma of 76Phyllachora isolates from herbarium and contemporary samples of
infected maize and other grass hosts. Exserohilum turcicum was
used as the outgroup.
Figure 3. Maximum likelihood phylogenetic tree based on ITS
sequence data from geographically representative isolates of the 5
genetic groups of maize and grass infecting Phyllachora from this
study and Phyllachora species available from GenBank. Specimens
highlighted in blue a contemporary isolates collect after 2015 and those
highlighted in orange are herbarium samples collected between 1905-1977.
Suppl. Figure 1 . Maximum likelihood phylogenetic tree based on
the ITS sequence data from the stroma of 169 Phyllachora isolates
from herbarium and contemporary samples of infected maize and other
grass hosts. Exserohilum turcicum was used as the outgroup.
Data Accessibility Statement. All DNA sequence data generated
by this project were deposited in Genbank as accessions
OL314402-OL314494 and OL342781-OL342949.
Acknowledgements . This research was supported in part by the
U.S. Department of Agriculture, Agricultural Research Service. FFAR-ROAR
grant to NK, CC, DT, MC, and DS with matching funds from Pioneer, The
National Corn Growers Association, The Illinois Corn Growers Improvement
Board, and Purdue University. Mention of trade names or commercial
products in this publication is solely for the purpose of providing
specific information and does not imply recommendation or endorsement by
the U.S. Department of Agriculture. USDA is an equal opportunity
provider and employer.