2.3 Sequence alignment, phylogenetic analysis and molecular identification
Sequences generated from this study were combined with sequences obtained from GenBank. Exserohilum turcicum and Cocoicola californica were selected as the outgroups. Sequence data was aligned and concatenated using MAFFT v.7 (mafft.cbrc.jp/alignment/server/index/html) using the G-INS-I model and manually inspected. The best fit partitioning schemes were determined using PartitionFinder (Lanfear et al. 2017) and used to build the phylogenies. Both single gene and concatenated gene sets were analyzed using a maximum likelihood (ML) analysis. The ML phylogenies were generated by RaxML under GTR model with gamma distributed rate heterogeneity with 1000 bootstrap replicates. Resulting trees were visualized with iTOL (Interactive Tree of Life) v.6 (https://itol.embl.de/) or MEGA. Sequences generated in this study were deposited in GenBank (Table 1)
RESULTS