Supplementary Figures
Fig. S1. (a) Frequency of isolation of L. cidri compared to the total yeast found. (b) Frequency of successful L. cidri isolation of the non-Saccharomyces yeast found.(c) n° of isolates per tree host.
Fig. S2. FACS analysis of L. cidri isolates. Number of cell vs propidium iodide intensity is shown. Haploid (n), diploid (2n), and tetraploid (4n). S. eubayanus strains were used as a control.
Figure S3. Genetic diversity across L. cidri populations. (a) Nucleotide diversity (π) in Chilean (SoAm) locations and the Australian (Aus) population. (b) Nucleotide diversity (π) in each locality. Altos de Lircay National Park (AL), Villarrica National Park (VI), Huilo-Huilo Biological Reserve (HH), Valdivian Coastal Reserve (VCR), Osorno Volcano (OV), Chiloé National Park (CH), Coyhaique National Reserve (CY), and Central Plateau of Tasmania (CP), Australia.
Fig. S4. Principal Component Analysis of the phenotypic data . Circles surround different populations (a) and localities (b) . South America population (SoAm), Australia population (Aus), Altos de Lircay National Park (AL), Villarrica National Park (VI), Huilo-Huilo Biological Reserve (HH), Valdivian Coastal Reserve (VCR), Osorno Volcano (OV), Chiloé National Park (CH), Coyhaique National Reserve (CY), Central Plateau of Tasmania (CP), Australia, and France (FR).
Fig. S5. Phenotypic diversity of SoAm L. cidri strains isolated from high and low altitude locations. (a) Fitness (µmax h-1) variation between Aus and SoAm population.(b) Fitness (µmax h-1) variation between High Altitude (HA= Altos de Lircay National Park, Villarrica National Park, Huilo-Huilo Biological Reserve, Osorno Volcano, Coyhaique National Reserve) and Low Altitude (LA= Valdivian Coastal Reserve and Chiloé National Park) isolation locations. (c) Fitness (µmax h-1) variation between HA and LA across conditions assessed. (*) indicate statistically significant differences according to the t-test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns = not significant).