Phenotypic diversity among isolates
To determine the phenotypic diversity across the L. cidriisolates, we estimated growth and biomass production in several
environmental conditions that represent different yeast habitats, either
in nature or in industrial settings. For this, we performed
high-throughput phenotyping in 96-well microculture plates as previously
described (Kessi-Perez et al., 2016). Briefly, cells were pre-cultivated
in 200 μL YNB medium without agitation at 20°C for 48 hours. For the
experimental run, each well was inoculated with ten μL of pre-inoculum
to an optical density (OD) of 0.03–0.1 in 200 μL of different media
compositions (Table S3). Culture OD was measured at 620 nm every 30
minutes for 96 hours. From these data, three parameters were estimated:
lag phase, growth rate (μmax), and maximum OD using the Growth Rates
software with default parameters (Hall et al., 2014).