Supplementary Figures
Fig. S1. (a) Frequency of isolation of L. cidri compared
to the total yeast found. (b) Frequency of successful L.
cidri isolation of the non-Saccharomyces yeast found.(c) n° of isolates per tree host.
Fig. S2. FACS analysis of L. cidri isolates. Number of
cell vs propidium iodide intensity is shown. Haploid (n), diploid (2n),
and tetraploid (4n). S. eubayanus strains were used as a control.
Figure S3. Genetic diversity across L. cidri populations.
(a) Nucleotide diversity (π) in Chilean (SoAm) locations and the
Australian (Aus) population. (b) Nucleotide diversity (π) in
each locality. Altos de Lircay National Park (AL), Villarrica National
Park (VI), Huilo-Huilo Biological Reserve (HH), Valdivian Coastal
Reserve (VCR), Osorno Volcano (OV), Chiloé National Park (CH), Coyhaique
National Reserve (CY), and Central Plateau of Tasmania (CP), Australia.
Fig. S4. Principal Component Analysis of the phenotypic
data . Circles surround different populations (a) and
localities (b) . South America population (SoAm), Australia
population (Aus), Altos de Lircay National Park (AL), Villarrica
National Park (VI), Huilo-Huilo Biological Reserve (HH), Valdivian
Coastal Reserve (VCR), Osorno Volcano (OV), Chiloé National Park (CH),
Coyhaique National Reserve (CY), Central Plateau of Tasmania (CP),
Australia, and France (FR).
Fig. S5. Phenotypic diversity of SoAm L. cidri strains
isolated from high and low altitude locations. (a) Fitness (µmax
h-1) variation between Aus and SoAm population.(b) Fitness (µmax h-1) variation between High
Altitude (HA= Altos de Lircay National Park, Villarrica National Park,
Huilo-Huilo Biological Reserve, Osorno Volcano, Coyhaique National
Reserve) and Low Altitude (LA= Valdivian Coastal Reserve and Chiloé
National Park) isolation locations. (c) Fitness (µmax
h-1) variation between HA and LA across conditions
assessed. (*) indicate statistically significant differences according
to the t-test (*p < 0.05; **p < 0.01; ***p
< 0.001; ****p < 0.0001; ns = not significant).