Cell culture
HEK (Human Embryonic Kidney) 293T cells (ATCC, CRL-321) and Vero E6 (Monkey African Green Kidney) (ATCC, CRL-1586) cells were cultured in Dulbecco’s modified Eagle medium (DMEM, PAN Biotech, P04-3500) supplemented with 10% fetal bovine serum (PAN Biotech, FBS standard, P30-3306) and %1 L-glutamine (200 mM; PAN Biotech, P04-80100 ) and 1% penicillin/streptomycin (Pan Biotech, P06-07100) at 5% (v/v) CO2, 37 °C incubator.