In Figure 5 , pseudovirus neutralization assay results were
plotted against PRA results (Wuhan type) to compare their
reliability. Plots showed linearity with a measure of certainty of
R2 = 0.7 (p= 0.0006) for pseudovirus neutralization
against PRA with isolated authentic virus (Figure 5A) . The
measure of certainty (R2) was decreased for
pseudovirus bearing Spike protein of Alpha and Betavariants against the PRA with Wuhan strain (Figure 5B and
5C ). Linearity test of pseudovirus bearing Spike protein ofAlpha variant was less significant for the comparison of
pseudovirus bearing Spike protein of Wuhan strain versus PRA
(R2 = 0.31, p= 0.048). In general, the pseudovirus
neutralization assay overestimated the samples with low neutralizing
activities determined by PRA. It might be less reliable for plasma
samples with low neutralizing capacity.