Figure 2. Viral entry assay in Vero E6 cell line. (A)Vero-E6 cells were inoculated with VSV-ΔG-Sdel21 pseudotyped virus. Cells were treated with hydroxychloroquine at 20 min before inoculation. After 24h, GFP signal detected under fluorescence microscope is shown in representative cellular images. (B) For quantification, GFP signal from 25 images per sample was analyzed by using Image J at 24h of post-inoculation. Values are presented as % of total population. Scale bar: 100µm. Data are represented as means of ±S.D. of at least three independent experiments. *p < 0.05; ****p < 0.0001 - using One-Way ANOVA with Bonferroni’s post-hoc test.