Cell culture
HEK (Human Embryonic Kidney) 293T cells (ATCC, CRL-321) and Vero E6
(Monkey African Green Kidney) (ATCC, CRL-1586) cells were cultured in
Dulbecco’s modified Eagle medium (DMEM, PAN Biotech, P04-3500)
supplemented with 10% fetal bovine serum (PAN Biotech, FBS standard,
P30-3306) and %1 L-glutamine (200 mM; PAN Biotech, P04-80100 ) and 1%
penicillin/streptomycin (Pan Biotech, P06-07100) at 5% (v/v)
CO2, 37 °C incubator.