Figure 1. Generation of VSV pseudotyped viruses bearing truncated SARS-CoV-2 Spike proteins. (A) Preparation of rVSV-ΔG-G* in BHK21/W1 cell line. (B) Packaging of rVSV-ΔG-Sdel21 in HEK293T cell line. (C) Infection of pseudovirus carrying GFP reporter and sequential passaging of pseudovirus to eliminate parental virus (rVSV-ΔG-G*) using plasmids encoding Sdel21 and T7 polymerase transfected Vero E6. (D)SARS-CoV-2 Spike pseudovirus neutralization assay using human convalescent plasma samples and GFP signal reading.