Western Blot (WB)
Western Blot analysis was performed to validate SARS-CoV-2 Spike protein
expression in HEK293T cells. Total protein was subjected to SDS-PAGE
followed by immunoblotting to nitrocellulose membranes (250 mA for 1.15
hours). Rabbit SARS-CoV-2 (2019-nCoV) Spike S1 monoclonal antibody
(40150-R007, SinoBiological) and a horseradish peroxidase-conjugated
secondary antibody (1/10,000) were used for detection. Antibodies were
used at 1/500 for Spike S1 subunit and at 1/10,000 for β-actin.