Immunocytochemistry (ICC)
Indirect immunofluorescence analysis was performed for cellular Spike
protein and ACE-2 protein detection. Vero E6 cells which are infected
with Spike pseudotyped VSV-ΔG virus were fixed with %4 Paraformaldehyde
(PFA) and stained with anti-Spike antibody (SinoBiological 2019-nCoV
Spike S1 Rabbit mAb, 40150-R007) (1:80 dilution), then incubated with
1:1000 diluted Alexa Fluor 568-conjugated goat anti-rabbit IgG secondary
antibody. ACE-2 protein was detected with anti-hAce2 (R&D Human Ace2,
AF933) (1:120 dilution), incubated with Alexa Fluor 568-conjugated
anti-goat IgG secondary antibody (1:500 dilution).