Figure 1. Generation of VSV pseudotyped viruses bearing
truncated SARS-CoV-2 Spike proteins. (A) Preparation of
rVSV-ΔG-G* in BHK21/W1 cell line. (B) Packaging of
rVSV-ΔG-Sdel21 in HEK293T cell line. (C) Infection of
pseudovirus carrying GFP reporter and sequential passaging of
pseudovirus to eliminate parental virus (rVSV-ΔG-G*) using plasmids
encoding Sdel21 and T7 polymerase transfected Vero E6. (D)SARS-CoV-2 Spike pseudovirus neutralization assay using human
convalescent plasma samples and GFP signal reading.