2.2. Development of the W/O emulsions
We developed CW solutions through the factorial combination of different
weights of the VO (i.e., 40 g, 50 g, 60 g) and the CW (0.75 g, 1.5 g,
2.25 g, 3.0 g). The CW was fully dissolved in the oil by heating and
intermittent stirring for 40 min in an oven set at 100°C. To develop the
oleogels the CW solutions were allowed to cool until achieving 25°C
(i.e., the cooling stage). For the oleogels formulated with 40 g of oil
the %CW varied between 1.84% and 6.98%, for the oleogels formulated
with 50 g of oil the %CW varied between 1.48% and 5.66%, and for
those formulated with 60 g of oil the %CW varied between 1.23% and
4.76%. Afterwards, the corresponding amount of deionized water (at
25°C) was added to make a total amount of 100 g. In this way, we
obtained systems with water to oleogel weight ratios of 40:60, 50:50,
and 60:40. With these systems we developed the emulsions by stirring for
60 seconds with an ultra-turrax
type homogenizer (RIVAL Immersion blender Model IB900. The Holmes Group,
Milford, MA) set at the higher speed (i.e., the emulsification stage).
The resulting emulsions at each of the water to oleogel ratios studied,
had CW concentrations of 0.75%, 1.5%, 2.25%, and 3%. The emulsions
developed were analyzed immediately and after 20 days of storage at
25°C.