2.4.3. Genetic differentiation between species
Overall and pairwise differentiation was assessed using the complete set of 5,702 SNPs by calculating FST (Weir and Cockerham 1984) using 10,000 bootstraps with the R package StAMPP v1.6.1 (Pembleton, Cogan, & Forster, 2013). To test whether hybridization and introgression resulted in reduced differentiation between species in sympatry, we first compared overall genetic differentiation betweenI. elegans and I. graellsii from allopatry with the overall genetic differentiation between I. elegans and I. graellsii from the Spanish hybrid region (including all individuals minus the F1 and F2 hybrids). Second, pairwise genetic differentiation among localities was calculated forI. elegans and I. graellsii , for all individuals before excluding the hybrids, and for all individuals minus the F1 and F2 hybrids.