2.4.3. Genetic differentiation between species
Overall and pairwise differentiation was assessed using the complete set
of 5,702 SNPs by calculating FST (Weir and Cockerham
1984) using 10,000 bootstraps with the R package StAMPP v1.6.1
(Pembleton, Cogan, & Forster, 2013). To test whether hybridization and
introgression resulted in reduced differentiation between species in
sympatry, we first compared overall genetic differentiation betweenI. elegans and I. graellsii from allopatry with the
overall genetic differentiation between I. elegans and I.
graellsii from the Spanish hybrid region (including all individuals
minus the F1 and F2 hybrids). Second,
pairwise genetic differentiation among localities was calculated forI. elegans and I. graellsii , for all individuals before
excluding the hybrids, and for all individuals minus the
F1 and F2 hybrids.