Region Species proportions Sampling locality, country N Year Latitude Longitude
Allopatry I. graellsii (100%) *Seyhouse, Algeria 7 (I. g) 2011 36.2827 7.2227
I. graellsii (100%) Salgados, Portugal 9 (I. g) 2007 37.097 -8.334
I. graellsii (100%) Cachadas, Spain 9 (I. g) 2015 42.2712 -8.5055
I. elegans (100%) Leuven, Belgium 6 (I. e) 2015 50.5341 4.4318
I. elegans (100%) Liverpool, UK 8 (I. e) 2008 53.2439 -2.584
I. elegans (100%) Vigueirat, France 10 (I. e) 2014 43.5311 4.3012
I. elegans (100%) Marais D’Orx, France 9 (I. e) 2015 43.3453 1.2359
I. elegans (100%) Saint Cyprien, France 10 (I. e) 2015 42.3823 3.0112
North-central hybrid region I. graellsii (99%) and I. elegans (1%) Mateo, Spain 10 (I. g) 2015 42.3036 -2.5154
I. graellsii (95%) and I. elegans (5%) Valpierre, Spain 10 (I. g) 2015 42.276 -2.5436
I. elegans (90%) and I. graellsii (10%) Villar, Spain 5 (I. e), 4 (I. g) 2015 42.242 -2.4216
I. elegans (84%) and I. graellsii (16%) Arreo, Spain 7 (I. e), 3 (I. g) 2008 42.4775 -2.5787
I. elegans (80%) and I. graellsii (20%) Perdiguero, Spain 5 (I. e), 4 (I. g) 2015 42.1718 -1.5846
I. elegans (64%) and I. graellsii (36%) Cañas, Spain 8 (I. e), 2 (I. g) 2015 42.2853 -2.2414
I. graellsii (51%) and I. elegans (49%) Valbornedo, Spain 8 (I. e), 2 (I. g) 2015 42.2448 -2-349
Norh-west hybrid region I. elegans (98%) and I. graellsii (2%) Doniños, Spain 10 (I. e) 2014 43.2927 -8.1855
I. elegans (98%) and I. graellsii (2%) Laxe, Spain 10 (I. e) 2014 43.6193 -8.1112
I. graellsii (95%) and I. elegans (5%) Louro, Spain 1 (I. e), 9 (I. g) 2013 42.6908 -8.6603
I. graellsii (100%) Xuño, Spain 10 (I. g) 2014 42.3733 -9.231
North-east Spain (Menorca) I. elegans (100%) Albufeira, Spain 9 (I. e) 2009 39.5647 4.1555
*Two samples removed after SNP calling due to possible hybridization with a third unknown species. ADMIXTURE analyses to test past hybridization the region are given in the Supplementary Figure S4. *Two samples removed after SNP calling due to possible hybridization with a third unknown species. ADMIXTURE analyses to test past hybridization the region are given in the Supplementary Figure S4. *Two samples removed after SNP calling due to possible hybridization with a third unknown species. ADMIXTURE analyses to test past hybridization the region are given in the Supplementary Figure S4. *Two samples removed after SNP calling due to possible hybridization with a third unknown species. ADMIXTURE analyses to test past hybridization the region are given in the Supplementary Figure S4. *Two samples removed after SNP calling due to possible hybridization with a third unknown species. ADMIXTURE analyses to test past hybridization the region are given in the Supplementary Figure S4. *Two samples removed after SNP calling due to possible hybridization with a third unknown species. ADMIXTURE analyses to test past hybridization the region are given in the Supplementary Figure S4. *Two samples removed after SNP calling due to possible hybridization with a third unknown species. ADMIXTURE analyses to test past hybridization the region are given in the Supplementary Figure S4.