Statistical experimental design and statistical analyses
A full factorial design at three levels (Modde v12.0 software, Umetrics,
Sweden) was used to investigate the effects of studied factors on the
yield and secretion of the recombinant protein. Studied factors were
KNO3, NAA and MeJA.
Statistical analyses were conducted with IBM SPSS Statistics 25
software. Normality of the data was assessed with Shapiro-Wilk’s test.
Unless otherwise described, one-way ANOVA was used together with Tukey
HSD as post-hoc test when homogeneity of variances allowed (Levene test
p>0.05). With data comprising unequal variances, Dunnett T3
test was used. Confidence level p<0.01 was used throughout the
data.