Measurement of mitochondria length
Cells plated ion 12 mm coverslips were incubated with 0.5 μM Mitotracker Red (Invitrogen) at 37 °C for 30 min, and then fixed with 4 % formaldehyde. The stained cells were mounted on glass slides using CYTOSEAL 60 mounting medium (Thermo Scientific). MitoTracker Red intensity was observed at 581 nm (excitation) using a laser scanning confocal microscope (Nikon). Quantification of the mitochondria length was performed on least 5 images using NIS-elements AR Analysis software (Nikon).