RNA interference
The siRNA specific to the human eEF2 and mouse eEF2, and the scrambled siRNA were prepared by Dharmacon. The following specific double strands were used: huma n, 5’-GGUGUUUGAUGCGAUCAUGAAUUTC-3’ (sense), 5’-GAAAUUCAUGAUCGC AUCAAACACCUU-3’ (anti-sense); mouse , 5’-GGUGU UUGAUGCGAUCAUGAAUUTC-3’ (sense), 5’-GAAAUUCA UGAUCGCAUCAAACACC UU-3’ (antisense). siRNAs were introduced into cells using RNAi MAX (Invitrogen) according to the manufacturer’s protocol.