Measurement of mitochondria length
Cells plated ion 12 mm coverslips were incubated with 0.5 μM Mitotracker
Red (Invitrogen) at 37 °C for 30 min, and then fixed with 4 %
formaldehyde. The stained cells were mounted on glass slides using
CYTOSEAL 60 mounting medium (Thermo Scientific). MitoTracker Red
intensity was observed at 581 nm (excitation) using a laser scanning
confocal microscope (Nikon). Quantification of the mitochondria length
was performed on least 5 images using NIS-elements AR Analysis software
(Nikon).