Cell cultures
Human breast cancer cell lines MCF7 and MDA-MB-231, glioblastoma LN229, human astroglia SVGp12, mouse embryonic fibroblast line MEF, and mouse melanoma cell line B16, were purchased from ATCC and maintained in Dulbeco modified Eagle’s medium (DMEM) (Sigma) supplemented with 10% (v/v) fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin.