RNA interference
The siRNA specific to the human eEF2 and mouse eEF2, and the scrambled
siRNA were prepared by Dharmacon. The following specific double strands
were used: huma n, 5’-GGUGUUUGAUGCGAUCAUGAAUUTC-3’ (sense),
5’-GAAAUUCAUGAUCGC AUCAAACACCUU-3’ (anti-sense); mouse , 5’-GGUGU
UUGAUGCGAUCAUGAAUUTC-3’ (sense), 5’-GAAAUUCA UGAUCGCAUCAAACACC UU-3’
(antisense). siRNAs were introduced into cells using RNAi MAX
(Invitrogen) according to the manufacturer’s protocol.