4.3 Strains, media, and culture conditions
LB medium and plates were used for E. coli cultivation. Kanamycin
and ampicillin were added at 100 mg L-1 when used
alone, 50 mg L-1 when combined. For plasmid
amplification and molecular cloning, we used E. coli DH5ɑ,
cultivated at 37℃, 220 r.p.m. For protein expression, E. coliBL21(DE3) strains (TIANGEN and TSINGKE) were used with regulations on
protein expression by lacO in the presence of different
concentrations of IPTG, respectively. Seed cultures were grown at 37℃,
220 r.p.m, and adjusted to OD600 of 0.6-1.0 for IPTG
induction. Since intrinsically disordered proteins were known to form
inclusion bodies at high expression levels and may cause the misfolding
of fused proteins, all of them were expressed at 16℃ and 220
r.p.m[49], while the light responses and enzymatic
reactivities were studied at room temperature or appropriate
temperatures.