Phylogenetic analyses
We used all Diestramima samples as well as the samples of
adjacent genera Diestrammena and Raphidophora in the
phylogenetic tree construction. For each fragment, DNA alignment was
conducted by MEGA7 software (Loytynoja & Goldman 2010; Kumar et
al . 2016). Saturation level of sequences was verified by Xia’s test
(Xia et al ., 2003) in dambe 7 (Xia, 2018). In PhyloSuite (Zhanget al . 2020), the best-fit evolutionary model GTR was derived
from ModelFinder (Kalyaanamoorthy et al . 2017), and the Maximum
likelihood (ML) analysis was performed in IQ-Tree (Nguyen et al .
2014) with 1000 ultrafast bootstrap replicates. Node support is shown as
posterior probabilities and bootstrap value, as directly estimated from
the majority rule consensus topology.