Ancestral range estimation
We defined the following six biogeographical areas of Diestramimaaccording to their known distributions and the zoogeographic regionalization of China (Zhang et al ., 2011): A. mountainous region of southern Yunnan (Baoshan city, Xishuangbanna Dai autonomous prefecture, south of Ailaoshan in Yuxi city, Puer city, Honghe Hani & Yi autonomous prefecture, west of Wenshan Zhuang & Miao autonomous prefecture); B. mountainous hilly region of Yunnan and Guangxi (east of Wenshan Zhuang & Miao autonomous prefecture, Baise city, Chongzuo city, Fangchenggang city, Nanning city); C. low mountainous hilly region of Guangxi, Guizhou and Hunan (Hechi city, Liuzhou city, Guilin city, south of Qiannan Buyi & Miao autonomous prefecture, east of Qiandongnan Miao & Dong autonomous prefecture, east of Hunan province); D. Sichuan basin (most of Chongqing city and east of Sichuan province); E. Jiangnan hilly region (southeast of Hunan, Xianning city in Hubei, most of Jiangxi, north of Fujian, most of Zhejiang); F. mountainous hilly region of Fujian and Guangdong (east of Guangxi province, north of Guangdong province, south of Fujian province).
Ancestral ranges were reconstructed using RASP 4.0 (Yu et al ., 2020) beta. As a guide tree for statistical dispersal-extinction-cladogenesis (S-DEC; Beaulieu et al ., 2013) analyses, we removed all outgroups and constructed a phylogenetic tree for the genus Diestramima . Modify and guide this tree according to the maximum credibility tree from the BEAST analysis. Ancestral areas were limited to no more than three areas. Statistical dispersal-vicariance (S-DIVA; Yu et al ., 2010) analysis were performed based on a sample of 1000 random post burn-in RASP trees and use the final tree. The maximum number of ancestral areas was limited to three.