2.1 Cell and Animal tissue samples
Animal tissue samples were provided by Zhengzhou zoo; CrandellRees
feline kidney cell (CRFK) cells were purchased from Procell Life
Science&Technology Co,.Ltd. In this study, CRFK were cultured in
Dulbecco’s modified Eagle’s medium (Gibco, USA) that had been
supplemented with 10% fetal bovine serum (HyClone, USA) and penicillin
streptomycin (Life Technologies, USA). The CRFK were cultured to
approximately 80% confluence and then infected with FPV HNZZ-2.