7. Diagnosis:
Most of the diagnostic methods of this virus are carried by the
identification of antigen in tissues (immunofluorescence) or
serologically by IgG and IgM antibody (ELISA) (Bastos et al., 2012). The
IgM capture ELISA method is carried out as a primary diagnostic tool,
but reverse transcription-polymerase chain reaction (RT-tests are now
accessible for identification of the CQV (Zhang et al., 2015; Ladner et
al., 2014). Accurate confirmation of the presence of
accuratevirus can only bedone through conventional RT-PCRthat is
followed by sequencing.However, an earlierstudy has been reported that
the CQVhas been isolated and then diagnosed by the next-generation
sequencing (NGS) method.NGS of agents causing idiopathic human
infections have been important in the detection of novel viruses(Whitmer
et al., 2018; Yadav et al., 2016). Recently, a precise anda very
sensitive multiplexed one-step RT-qPCR were assessed for CQV
identification inthe cell supernatants and mouse tissues (Naveca et al.,
2017).