7. Diagnosis:
Most of the diagnostic methods of this virus are carried by the identification of antigen in tissues (immunofluorescence) or serologically by IgG and IgM antibody (ELISA) (Bastos et al., 2012). The IgM capture ELISA method is carried out as a primary diagnostic tool, but reverse transcription-polymerase chain reaction (RT-tests are now accessible for identification of the CQV (Zhang et al., 2015; Ladner et al., 2014). Accurate confirmation of the presence of accuratevirus can only bedone through conventional RT-PCRthat is followed by sequencing.However, an earlierstudy has been reported that the CQVhas been isolated and then diagnosed by the next-generation sequencing (NGS) method.NGS of agents causing idiopathic human infections have been important in the detection of novel viruses(Whitmer et al., 2018; Yadav et al., 2016). Recently, a precise anda very sensitive multiplexed one-step RT-qPCR were assessed for CQV identification inthe cell supernatants and mouse tissues (Naveca et al., 2017).