3.3 │ High proportion of false positives in semens directly detected by ELISA
Semens were detected directly using ELISA, and the results showed that 10 samples were positive, but only 6 corresponding samples showed positive from virus isolation in plasmas or semen (accurucy rate 42.86% (6/14)). At least 4 samples showed negtive in the ELISA were false negtives. Therefore, the remaining 4 positive semen sample in the ELISA were false positives (mistaken rate 8.69%) (Table 2).