3.4 │ Identification of Avian leukosis virus isolated from roosters, hens and albumens
The positive samples from roosters, hens, and albumens were went through virus isolation and sequence analysis, and were classified into ALV-J by the gp85 gene and phylogenetic tree analysis (Figure 1). IFA also showed positive with the monoclonal antibody JE9 (Figure 2). However, these strains are located in a new branch of the subgroup J, and do not show obvious homology through the comparison with domestic and foreign referential sequences (Figure 1).