Figure 1. The phylogenetic tree of gp85 gene in the isolates and the referential strains
The phylogenetic tree is generated using the neighbor-joining method using MEGA5.1 software (bootstrap method with 1000 replicates). ALV-A to E, K and J were divided and marked on the right. The source of ALV-J reference strains is in parentheses. Purple rhombus represent the strains were isolated in the plasmas of the rooster. Green triangles represent the strains were isolated in the semens of the rooster. The other strains in this study are also highlighted by the red figure. The sources of reference and isolated strains are marked after each strain. The following indicates the abbreviations used for country names: USA=United States, UK=United Kingdom, CHN=China, RUS=Russia, EGY=Egypt, NGA=Nigeria.
Figure 2. The results of immunofluorescence assay on the DF1 cells with JE9 monoclonal antibody. (A) Negative control, 100×; (B) DF-1 cells infected with semens, 100×.