Taxon sampling and DNA extraction
In this study, single individuals of 28 typhlocybine species were newly
sequenced, including one representative of Alebrini, three from
Dikraneurini, three from Zyginellini and 21 from Typhlocybini. Vouchers
are deposited at the Institute of Entomology, Guizhou University,
Guiyang, China (GUGC). Nineteen additional mitogenome sequences were
obtained from
GenBank.
The 47 mitogenomes cover all currently recognized typhlocybine tribes
(sensu Dworakowska, 1979). Based on results of prior analyses of
Membracoidea, we selected representatives of Mileewinae (three species),
Cicadellinae (five species) and
Evacanthinae
(two species) as outgroups (Balme, 2007; Takiya, 2007;
Dietrich
et al., 2010; Dietrich, 2013b). Included taxa including voucher number,
GenBank accession number, and collection locality are listed in Table
S2. All specimens were identified to species before DNA extraction.
Genomic DNA was extracted from the legs and from the thoracic muscle
tissue using the DNeasy Blood and Tissue kit (Qiagen, Germany) following
the animal tissue protocol.