Mutation of D201G near the receptor binding site significantly drive antigenic drift of circulating H9N2 subtype avian influenza virus

Short title: Antigenic drift site of H9N2 AIV

Jing Xia^1,2¶, Yu-Wen Luo^1,2¶, Meng-Yi Dong^1,2, Yong-Xin Li^1,2, An-Dong Wang^1,2, Nian-Ling Li^1,2, Yu-Xi Shen^1,2, Shu-Yun Li^1,2, Min Cui^1,2, Xin-Feng Han^1,2, Song-Cheng Yu³, Min Li^4*, Yong Huang^1,2*

1. Preventive of Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.

2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.

3. Meishan Dongpo District Agricultural and Rural Burean, Livestock station, Meishan 620010, Sichuan, China

4. Animal disease prevention and control center of Chengdu city, Chengdu 610041, Sichuan, China

^¶ These authors contributed equally to this work

* Correspondence:

Yong Huang, E-mail: hyong601@163.com

Min Li, E-mail: 350360849@qq.com

Jing Xia: 14551@sicau.edu.cn xiajing1028@163.com

Yu-Wen Luo: Yuwen_Luo@126.com

Meng-Yi Dong: 18200356764@163.com

Yong-Xin Li: 41984561@qq.com

An-Dong Wang: antone@stu.sicau.edu.cn

Nian-Ling Li: linianling123@163.com

Yu-Xi Shen: shenyuxi1019@163.com

Shu-Yun Li: lishuyun607@163.com

Min Cui: cuimintracy@163.com

Xin-Feng Han: hanxinf@163.com

Song-Cheng Yu: 853769133@qq.com