T cell enrichment
Using Sepax C-pro, the fresh leukopak was washed and concentrated using CliniMACS PBS/EDTA Buffer (Miltenyi Biotech) supplemented with 0.1% HSA and then incubated with antibodies specific to CD4 and CD8 (Miltenyi Biotec) at room temperature for 30 minutes. Labeled leukopak cells were then transferred on CliniMACS Plus (Miltenyi Biotech) and positive fraction of flow through was collected according to manufacturers’ instructions.