Human Interferon-gamma (IFN-ɣ)
TCR-T cells were incubated with MAGE-B2 peptide-loaded T2 Luc cells at
various E:T ratios for 24 hours in CTS OpTmizer T Cell Expansion SFM
(Gibco) with 2.5% CTS Immune Cell SR (Gibco) at 1e6 cells/mL.
Supernatant was collected and analyzed for IFN-ɣ using AlphaLISA Human
Interferon-gamma Kit (PerkinElmer) according to manufacturer’s manual.
Sample and standard data was collected using EnVision plate reader
(PerkimElmer).