Figure 4: Thermal and chemical denaturation studies. (A) MRE at 222 nm at temperatures from 20 °C to 90°C at rates of 0.2 °C/min and 1 °C/min , measured by CD. (B) Thermal kinetics demonstrating fraction folded during 2 hour incubations at different temperatures (68 °C to 87.5 °C), measured by CD. (C) DSC showing two-transition unfolding ofTf C. (D) MRE at 222 nm at varying urea (0 M to 8 M) and GdmCl (0 M to 6 M) concentrations, measured by CD. (E) Chemical kinetics demonstrating fraction folded during 2 h incubations at different concentrations of GdmCl (5 M to 7 M), measured by CD.