Accessory gland size changes during recovery
Measurements were done on glands dissected from virgin males, as a
previous mating and the accompanying transfer of seminal fluid proteins
reduces AG size (Ruhmann et al., 2016). We dissected glands from males
20-24 hours, two, four and six days old and followed procedures as
carried out in (Ruhmann et al., 2016). We also measured wing length as
an indicator of male body size to control for the allometric
relationship between both factors (Shingleton et al., 2007). We measured
both AGs and wings for each male and the mean was used for the analysis.
In total, the AGs and wings of 15-26 males per treatment and day were
measured.