3.1. HMGB1 A Box inhibits macrophage infiltration in SN.
In previous studies, we detected that T cell infiltration in SN could be
significantly inhibited by HMGB1 A Box, and in vitro experiments
confirmed that midbrain microglia promoted Th17 differentiation.
However, the proportion of microglia promoting Th17 differentiationin vitro was significantly lower than that of MPTP model mice.
Therefore, we assume that peripheral antigen-presenting cells may also
contribute to Th17 differentiation in SN. In this study, we detected
CD45+F4/80+ macrophages and
CD45+CD11c+ DCs in the SN of MPTP
mice, and found that macrophages were outside the blood vessels or
lymphatic vessels marked by CD31-positive endothelial cells, this
indicates that macrophages are infiltrating the parenchyma of the SN,
and HMGB1 A Box significantly inhibited this process (Fig. 1A). However,
although MPTP also induced a certain increase in
CD45+CD11c+ DCs, they were localized
inside the blood vessels rather than the parenchyma (Fig. 1B).