2.6. Adoptive transfer of CD3+ T cells
The spleen-derived GFP+ CD3+ T cells
obtained by magnetic bead sorting were treated with or without 1 ug/mL
CXCR4 neutralizing antibody and HMGB1 A Box for 24 h in vitro. Cells
were then collected and transfused back into an 8-10 week old wild-type
C57BL6J male mice with a source of cells. The recipients were then given
daily intraperitoneal injection of MPTP 20 mg/kg for 7 days. After the
mice were anesthetized with isoflurane on the 8th day, the mice were
perfused with PBS and paraformaldehyde successively, and the mouse
brains were taken and serially sectioned at the SN. CD3 and GFP
fluorescence were observed under a microscope.