2.1.3. Optimization of protein
We optimized the separated structure by MOE molecular viewer 2013. a quick receptor optimization was used and probable defects were discovered and amino acids were localized on active sites of this protein by stereochemical estimations. Finally, the last structure was the most stable conformer with least energy level. 10The results of optimization (physicochemical properties, changes in physiological environments and effects of different parameters) are described in conclusions with details.