Results
In this study we performed transcriptome sequencing of individuals from two ecotypes of stickleback (saltwater and freshwater) being exposed to abrupt changes in salinity compared to their native environments. To investigate their overall response, we analysed four different comparisons (outlined in Table 1): one for each ecotype, one between ecotypes in their control experimental salinity and one aimed at finding transcripts that were equally expressed within ecotype regardless of salinity, through gene expression patterns.