2.3.3 Proximate composition
The crude protein content was determined according to AACC method 46-30.01 using a combustion nitrogen analyzer (FP-628 CAN, LECO Corp., Saint Joseph, MI, USA) with a nitrogen conversion factor of 6.25 (AACC, 2010). The content of ash was determined using 2 g of sample in a muffle furnace at 600℃ for a 2-h period according to AOAC method 943.05 (AOAC, 2005). The crude lipid was measured using the Goldfisch apparatus according to AOAC method 920.39 (AOAC, 2005). Measurements were made in triplicate on each of the replicate batches (n=3) and reported as the mean ± one standard deviation.