2.4 Hi-C assisted assembly
To obtain chromosome-level genome, we used (High-throughput/resolution chromosome conformation capture) Hi-C technology to assist assembly. The eggs were treated by paraformaldehyde to fixed DNA conformation for 10 mins and stopped crosslinking by 2.5 M glycine for 20 mins. Crosslink DNAs were cut with restriction enzyme and produced fill ends with biotin, which were used to build library and subsequent sequencing via Illumina platform. High quality clean data 58.615 Gb (read length: 150 bp) was generated after sequencing and filtering, then used to preliminary assembly by applying 3D-DNA pipeline using default parameters. Using Juicer to build the chromosome interaction map and using Juicebox to visually correct it for finding mistakes of contig sequence, direction or assembly error inside contig.