2.4 Hi-C assisted assembly
To obtain chromosome-level genome, we used (High-throughput/resolution
chromosome conformation capture) Hi-C technology to assist assembly. The
eggs were treated by paraformaldehyde to fixed DNA conformation for 10
mins and stopped crosslinking by 2.5 M glycine for 20 mins. Crosslink
DNAs were cut with restriction enzyme and produced fill ends with
biotin, which were used to build library and subsequent sequencing via
Illumina platform. High quality clean data 58.615 Gb (read length: 150
bp) was generated after sequencing and filtering, then used to
preliminary assembly by applying 3D-DNA pipeline using default
parameters. Using Juicer to build the chromosome interaction map and
using Juicebox to visually correct it for finding mistakes of contig
sequence, direction or assembly error inside contig.