Supporting Information
Figure S1. (A) Root and leaf ion concentrations (µg/g) and(B) ion translocation ratios (leaf [X] / root [X]) of
A1(c+), T6(c-) and Col-0 A.
thaliana plants cultivated in hydroponics under control (pH 5.7), high
pH (pH 8.3) and bic (10 mM NaHCO3, pH 8.3) conditions
for 10 days. Letters indicate significant differences between treatments
for each accession (t-test, p < 0.05).
Figure S2. (A) Growth (rosette diameter, mm) and fitness
(silique number) correlation and stats. (B) Bolting time and
fitness correlation and stats.
Figure S3. (A) Aerial and root biomass (dry weight, g) of
A1(c+), T6(c-),Col-0(WT) and 18 T-DNA knockout mutants of the candidate
genes grown hydroponically under control (1/2 Hoagland Solution (HS), pH
5.9) and bic stress (1/2 HS + 10 mM NaHCO3, pH 8.3) for
10 days. NP=no plant (no germination in phytoagar); D=dead plants.(B) Fitness (silique number) of A1(c+),
T6(c-), Col-0(WT) and 18 T-DNA knockout
mutants cultivated in natural carbonated soil. NP=no plant (no
germination in carbonated soil). Letters indicate significant
differences between treatment (p < 0.05, t-test) and numbers
or asterisks indicate significant differences respect the wild-type
Col-0 (p < 0.05, Dunnet’s test).
Figure S4. SNP changes detected in the (A) AHH
(AT4G20070), (B) PCH1 (AT2G16365), and (C) INV-E
(AT5G22510) genes of A1(c+) and T6(c-).
Missense variants are marked in red.
Dataset S1. Plant materials. A. thaliana natural demes
information, T-DNA lines of 18 candidate genes and primers used for qPCR
validation.
Dataset S2 . Phenomic and ionomic statistics from hydroponic and
carbonated soil cultivations.
Dataset S3 . DEGs from A1(c+) and
T6(c-) leaf and root transcriptomic analysis.
Dataset S4. GO terms, KEGG pathways, and STRING output from
root transcriptomics.
Dataset S5. Fitness and screening of A1xT6 progeny under
natural carbonated soil.
Dataset S6. BSA-Seq outliers (1119 genes) and GO terms of
outliers from Chromosome 5.
Dataset S7. A1(c+) vs T6(c-)allele frequency comparison (977 genes).
Dataset S8. Input gene lists for genomic and transcriptomic
association analysis.
Dataset S9. Gene expression and SNP changes of the 18 putative
candidate genes.