Results and discussion
Natural variation of wild species provides a relevant complementary resource to discover novel gene functions. It has been proven thatA. thaliana demes from the north-east of Spain harbor high genetic diversity [35] and they have a great potential for studying traits related to adaptation (e.g. [36]) and allelic variants that specifically interact with the environment (e.g. [37]). Teréset al. [5] selected two demes with contrasting phenotypes in soil carbonate tolerance, A1(c+) and T6(c-), and performed several studies to characterize the physiological mechanisms activated by these demes under carbonate and iron-deficiency stress.