Supporting Information
Figure S1. (A) Root and leaf ion concentrations (µg/g) and(B) ion translocation ratios (leaf [X] / root [X]) of A1(c+), T6(c-) and Col-0 A. thaliana plants cultivated in hydroponics under control (pH 5.7), high pH (pH 8.3) and bic (10 mM NaHCO3, pH 8.3) conditions for 10 days. Letters indicate significant differences between treatments for each accession (t-test, p < 0.05).
Figure S2. (A) Growth (rosette diameter, mm) and fitness (silique number) correlation and stats. (B) Bolting time and fitness correlation and stats.
Figure S3. (A) Aerial and root biomass (dry weight, g) of A1(c+), T6(c-),Col-0(WT) and 18 T-DNA knockout mutants of the candidate genes grown hydroponically under control (1/2 Hoagland Solution (HS), pH 5.9) and bic stress (1/2 HS + 10 mM NaHCO3, pH 8.3) for 10 days. NP=no plant (no germination in phytoagar); D=dead plants.(B) Fitness (silique number) of A1(c+), T6(c-), Col-0(WT) and 18 T-DNA knockout mutants cultivated in natural carbonated soil. NP=no plant (no germination in carbonated soil). Letters indicate significant differences between treatment (p < 0.05, t-test) and numbers or asterisks indicate significant differences respect the wild-type Col-0 (p < 0.05, Dunnet’s test).
Figure S4. SNP changes detected in the (A) AHH (AT4G20070), (B) PCH1 (AT2G16365), and (C) INV-E (AT5G22510) genes of A1(c+) and T6(c-). Missense variants are marked in red.
Dataset S1. Plant materials. A. thaliana natural demes information, T-DNA lines of 18 candidate genes and primers used for qPCR validation.
Dataset S2 . Phenomic and ionomic statistics from hydroponic and carbonated soil cultivations.
Dataset S3 . DEGs from A1(c+) and T6(c-) leaf and root transcriptomic analysis.
Dataset S4. GO terms, KEGG pathways, and STRING output from root transcriptomics.
Dataset S5. Fitness and screening of A1xT6 progeny under natural carbonated soil.
Dataset S6. BSA-Seq outliers (1119 genes) and GO terms of outliers from Chromosome 5.
Dataset S7. A1(c+) vs T6(c-)allele frequency comparison (977 genes).
Dataset S8. Input gene lists for genomic and transcriptomic association analysis.
Dataset S9. Gene expression and SNP changes of the 18 putative candidate genes.