ABSTRACT

Objective : To quantify the transplacental transmission of SARS-CoV-2 and antibodies in pregnant women and their new-borns according to the trimester of maternal infection.

Design : This was a prospective observational multicentre study including pregnant women with positivity for SARS-CoV-2 by reverse transcription–polymerase chain reaction (RT‒PCR) or positivity by serology from April to December 2020. The study was designed to perform a systematic collection of mother/new-born dyad samples at birth.

Setting : Eleven maternity wards in Eastern France

Population : Pregnant women with confirmed COVID-19 infection during pregnancy and their new-borns.

Methods : The SARS-CoV-2 viral load was measured by RT‒PCR. IgG and IgM antibodies to the receptor-binding domain of the SARS-CoV-2 spike protein were measured by enzyme-linked immunosorbent assay. Antibody concentrations and transplacental transfer ratios were analysed according to the term of maternal infection.

Main Outcome Measure: SARS-CoV-2 viral load in maternal plasma or respiratory fluids and umbilical cord plasma and quantification of anti-SARS-CoV-2 antibody transfer.

Results: Among 165 dyads enrolled, one congenital infection was confirmed (n=1 (0.63%) IC_95% [0.02%; 3.48%]). Among 165 maternal sera tested, 107 (64.8%) were positive for IgG with a concentration > 25.6 BAU/ml. The average placental transfer ratio was 1.27 (95% CI [0.69–2.89]). The transfer ratio increased with increasing time between the onset of maternal infection and delivery (P value = 0.0001).

Conclusions: We confirmed very low SARS-CoV-2 transplacental transmission of less than 1%. Transmission of antibodies is more likely when the infection occurs in the first or second trimester of pregnancy.