Grasshopper DNA extraction and sequencing
We collected samples from multiple populations from either side of theP. pedestris hybrid zone. For long-term storage, hindlegs were
kept in pure ethanol at 4℃. After equilibrating these in deionised water
on ice for ten minutes with their exoskeleton cut open, we extracted
whole genomic DNA using a Blood & Tissue Kit (Qiagen, Manchester, UK).
TruSeq sequencing libraries were generated by, and sequencing was
carried out at the Bart’s and the London Genome Centre on the NextSeq
500 platform generating paired-end reads of 76 nt length. The sequencing
coverages obtained ranged from 5.6% to 35.6% with a median of 7.4%.