Pre-clinical testing in healthy dogs
A small pre-clinical trial was conducted on four healthy dogs to preliminary assess the feasibility and local tolerability of normal skin to both doses (50 and 100 HEP/mL). We hypothesised that if skin irritation occurred in healthy skin, a more pronounced reaction would be expected in atopic skin, so lower doses would have to be considered. Owners were asked to perform a single 12-hour application of both doses in side-by-side patches, following the protocol described below. Both doses were well-tolerated, and the protocol was deemed feasible.