Allergen-based formulation
LETIPharma S.L.U. has fully supplied the lyophilised mite-allergen
extracts required (Dermatophagoides farinae ,Dermatophagoides pteronyssinus, Acarus siro , Tyrophagus
putrescentiae , Lepidogliphus destructor ). Glycerine was used as
a solvent, given its ability to retard allergen potency loss. A
tailor-made polyethylene glycol-based vehicle was specially designed for
this study, given its biocompatibility, well-established safety,
bioavailability, and stabilising, penetration enhancing and
controlled-release carrier effects40. A dose of 100
HEP/mL was chosen as it induced the strongest therapeutic effect and
achieved statistical significance in a double-blind, placebo-controlled,
dose-escalation human trial35. Nevertheless, a lower
dose (50 HEP/mL) was selected for the first application. Allergen-based
formulations were custom-produced for each dog every two-weeks,
according to their IgE serology panel.