Ethics
The project was approved for ethical standards by Comissão de Ética e Bem-Estar Animal (016/2020), Faculty of Veterinary Medicine, University of Lisbon, Portugal. All owners gave written informed consent for study participation and were free to withdraw at any time. Owner’s confidentially was ensured by a system of assigning a number to each dog, concealing the public disclosure of personal details. All procedures were conducted according to national legislation and animal welfare standards.