Allergen-based formulation
LETIPharma S.L.U. has fully supplied the lyophilised mite-allergen extracts required (Dermatophagoides farinae ,Dermatophagoides pteronyssinus, Acarus siro , Tyrophagus putrescentiae , Lepidogliphus destructor ). Glycerine was used as a solvent, given its ability to retard allergen potency loss. A tailor-made polyethylene glycol-based vehicle was specially designed for this study, given its biocompatibility, well-established safety, bioavailability, and stabilising, penetration enhancing and controlled-release carrier effects40. A dose of 100 HEP/mL was chosen as it induced the strongest therapeutic effect and achieved statistical significance in a double-blind, placebo-controlled, dose-escalation human trial35. Nevertheless, a lower dose (50 HEP/mL) was selected for the first application. Allergen-based formulations were custom-produced for each dog every two-weeks, according to their IgE serology panel.