Pre-clinical testing in healthy dogs
A small pre-clinical trial was conducted on four healthy dogs to
preliminary assess the feasibility and local tolerability of normal skin
to both doses (50 and 100 HEP/mL). We hypothesised that if skin
irritation occurred in healthy skin, a more pronounced reaction would be
expected in atopic skin, so lower doses would have to be considered.
Owners were asked to perform a single 12-hour application of both doses
in side-by-side patches, following the protocol described below. Both
doses were well-tolerated, and the protocol was deemed feasible.