Figure Legends
Figure 1 . Study design and differential gene expression of
histamine receptors. a. The study design used for isolation and
sorting of B-cells clones for gene expression analysis and the data
analysis workplan for identification and prioritization of GPCRs.b. Mutually exclusive expression profile of HRH1 andHRH2 in both unstimulated and BCR-stimulated samples. The dotted
box represents the threshold within which samples were considered as
double negative, i.e., HRH1- /HRH2- . c . Volcano
plots to highlight differentially expressed GPCR genes in HRH1+vs HRH2+ samples in both unstimulated and BCR-stimulated samples.
The red dots represent significantly differentially expressed genes (p
< 0.05 & log-foldchange > 0.5).
Figure 2 . Differentially expressed GPCRs in HRH1+ vsHRH2+ samples. a. Heatmap showing expression profile of
differentially expressed GPCR genes in all the BCR-stimulated samples,
including double negative samples. The top two panels represent theHRH1+ and HRH2+ status of each sample. b. The
boxplot showing topmost significantly differentially expressed GPCR
genes in (p < 0.001) in BCR-stimulated samples. c.Heatmap showing expression profile of differentially expressed GPCR
genes in all the unstimulated samples. The annotations are same as
Figure2a. d. The boxplot showing topmost significantly
differentially expressed GPCR genes in (p < 0.001) in
unstimulated samples.
Conflict of Interest: Authors declare no conflict of interest.
Author Contribution: IC: Writing, review & editing, formal
analysis; AK: Writing, review &editing, software and formal analysis;
PS, LB, SRS: review & editing; MY: formal analysis; KN: supervision,
review & editing; WvdV: review & editing, conceptualization, method,
provide data; CAA and MA: review & editing, conceptualization,
supervision