Figure 3. Comparison of results published by Finke & Suttle (2019) to those generated by the vAMPirus workflow for the same virus amplicon sequence dataset. The significant positive relationship between salinity and viral diversity (Shannon diversity (H ) and richness) reported by Finke and Suttle (2019, panels I and II) was reproduced by vAMPirus (panels III-VI). Panels I and II display original results from Finke and Suttle (2019), based on 97% amino acid similarity OTUs. Panels III, IV, V, and VI include vAMPirus 97% pcASV results. Figures from Finke and Suttle (2019) and vAMPirus analyses were slightly modified for readability. For the vAMPirus analysis, which performs comparisons among categorical sample groups set by the user, all samples were assigned to either ‘high’ (>27.5) or ‘low’ (<27.5) salinity (practical salinity units; psu) groups. Figures from Finke and Suttle (2019) reprinted with permission from authors.