Pre-experimental results
By performing germination tests after immersion in different concentrations of GA3 and H2O2 solutions, we found that the final germination rate (Fig 1a) and germination potential (Fig 1b) of the seeds of V. negundo var. heterophylla were greatest after treatment with a GA3 pre-treatment solution at a concentration of 1‰, with the final germination rate of 68.60% and the germination energy of 22, and thus in pre-treatment solution was most favorable for seed germination of V. negundo var.