Cell culture
FaDu cancer cells were purchased from American Type Culture Collection
(ATCC, Manassas, VA, USA) and maintained by Eagle’s Minimum Essential
Medium (Gibco, Grand Island, NY, USA) supplemented with 15% foetal
bovine serum in a 5% CO2 incubator at 37°C. hBMSCs were administered
with complete mesenchymal stem cell culture medium (Gibco, Grand Island,
NY, USA) containing 10% fetal bovine serum and 1% double antibody
(penicillin mixture) and cultured in a 5% CO2 incubator at 37°C.