Isolation and identification of exosomes
Exosome particle size and concentration were measured using nanoparticle
tracking analysis (NTA) at Viva Cell Biosciences with Zeta View PMX 110
(Particle Metrix, Meerbusch, Germany) and corresponding software Zeta
View 8.04.02. Isolated exosome samples were appropriately diluted using
1X PBS buffer (Biological Industries, Israel) to measure the particle
size and concentration. NTA measurement was recorded and analyzed at 11
positions. The Zeta View system was calibrated using 110 nm polystyrene
particles. Temperature was maintained around 23°C and 30°C. Photograph
of exosomes was took with electron microscope.