miR-21 was up-regulated in hypopharyngeal cancer tissue and FaDu
cells.
We firstly analyzed the expression level of miR-21 in hypopharyngeal
cancer tissues samples and hypopharyngeal cancer cell line FaDu cells,
with a normal cell line (293T cell line) as control. qRT-PCR showed that
miR-21 expression was both significantly increased in hypopharyngeal
cancer tissues samples (Fig. 1a) and FaDu cells compared to control
(Fig. 1b).