Isolation and identification of exosomes
Exosome particle size and concentration were measured using nanoparticle tracking analysis (NTA) at Viva Cell Biosciences with Zeta View PMX 110 (Particle Metrix, Meerbusch, Germany) and corresponding software Zeta View 8.04.02. Isolated exosome samples were appropriately diluted using 1X PBS buffer (Biological Industries, Israel) to measure the particle size and concentration. NTA measurement was recorded and analyzed at 11 positions. The Zeta View system was calibrated using 110 nm polystyrene particles. Temperature was maintained around 23°C and 30°C. Photograph of exosomes was took with electron microscope.