Flow cytometry
Collect and fix cell sample, add 500μL staining buffer, 10uLRNase A, 25 μL propidium iodide solution, then put in dark at 37 ℃ for 30min. Flow detection was conducted within 24h after dyeing. Use flow cytometer (Beckman, USA) to detect red fluorescence at the excitation wavelength of 488nm, corresponding to the flow cytometer FL2 detection channel, and detect light scattering at the same time. Subsequently, the cell cycle was analyzed with FLOWJO software.