Method description
We performed effusion of 20-30 ml of the patient’s whole blood in a
syringe (50ml) with 5ml of anticoagulant (ACD-A). The volume of effusion
depended on the level of hematocrit. This was the most important
limiting factor, as the maximum dilution was allowed up to 300ml as
required by the ECP system manufacturer. The obtained whole blood was
injected into a photopheresis bag (MacoPharma, France) adding 5 ml of
8-MOP (Metoxsalene S.A.L.F, Italy) and incubated in the dark at room
temperature for 1 hour. Then sterile NaCl 0.9% solution was added into
the bag to 300 ml. The resulting cell product was irradiated with
ultraviolet light in the MacoGenic G2 (MacoPharma, France). After
phototreatment, the cell product was reinfused to the patient.