6. Conclusion
In this study, we have established and validated a method to determine the concentration of TGC in serum and CSF. This method could achieve the quantification accurately in 2.5 min. We quantified the bio-samples from 12 MDRAB CNSI patients treated with TGC to evaluate CSF-to-serum ratio of TGC at steady-state trough concentration. And the correlation between the concentrations of TGC in CSF and serum at steady-state was demonstrated to be positive. CNSI also did not show the potential to increase the penetration ability of TGC to CSF.