2.5.3 Precision and accuracy
Intra-day, inter-day precision were evaluated by analyzing QCs (n=5) at
low, medium and high concentrations (10 ng/mL, 400 ng/mL, 800 ng/mL) on
the same day or 3 different days respectively. The precision was defined
as the relative standard deviation (RSD) of QC sample concentrations
determined at 5 replicates. The accuracy was assessed as the percentage
to the nominal concentration (%) by analyzing 5 repetitive QCs at each
concentration. The mean values should be within 15% of the nominal
value except low concentration, which should not deviate more than 20%.