2.6 Application
This study was approved by the ethics committee of the first affiliated hospital of Harbin medical university. 12 patients were diagnosed of MDRAB CNSI (with positive CSF culture of MDRAB for twice) after undergoing a neurosurgical procedure. They were treated with 100 mg TGC as a loading dose, and then the dosage followed the scheme of 50 mg/12h. The CSF samples were collected through lumbar puncture in vacuum collection tube before the start of daily administration after 7 days of treatment. And the blood samples were collected at the same point. Blood samples were centrifuged at 4000 rpm for 5 min (4°C) to separate the serum. The serum and CSF samples were all stored at –80 °C immediately.