2.6 Application
This study was approved by the ethics committee of the first affiliated
hospital of Harbin medical university. 12 patients were diagnosed of
MDRAB CNSI (with positive CSF culture of MDRAB for twice) after
undergoing a neurosurgical procedure. They were treated with 100 mg TGC
as a loading dose, and then the dosage followed the scheme of 50 mg/12h.
The CSF samples were collected through lumbar puncture in vacuum
collection tube before the start of daily administration after 7 days of
treatment. And the blood samples were collected at the same point. Blood
samples were centrifuged at 4000 rpm for 5 min (4°C) to separate the
serum. The serum and CSF samples were all stored at –80 °C immediately.