2.10. Drug screening analysis and biosensor sensitivity study
The drug toxicity of the LOC platform was determined by incubating it with different concentrations of diclofenac (DF) (1, 10, 100, 200, 400, 800, and 1000 μM) (Sigma) for 48 h. After treatment, the cell viability of the LOC was assessed using the CCK-8 assay, and measurements were performed calorimetrically. To determine the biosensor sensitivity, CA measurement of the drug-treated LOC was performed.