2.3. Prediction of potential coding capacity of mitogenome
Mitogenome data of Apis mellifera was analyzed in ORFFinder (Open Reading Frame Finder, NCBI) in order to predict additional gene regions which are able to encode proteins different than known 13 OXPHOS proteins. Search parameters were adjusted as “invertebrate mitochondrial genetic code” and “”ATG” and “alternative initiation codons”. Potential protein encoding sequences were then verified using regular BLASTP.