2.3. Prediction of potential coding capacity of mitogenome
Mitogenome data of Apis mellifera was analyzed in ORFFinder (Open
Reading Frame Finder, NCBI) in order to predict additional gene regions
which are able to encode proteins different than known 13 OXPHOS
proteins. Search parameters were adjusted as “invertebrate
mitochondrial genetic code” and “”ATG” and “alternative initiation
codons”. Potential protein encoding sequences were then verified using
regular BLASTP.