2. 1. Sample selection
Apis mellifera RNA-seq data (SRX5798518) and Apis mellifera sahariensis data (NC_035883) as reference mitogenomes were downloaded from NCBI database. The RNA-seq data was generated using the brain tissues of Apis mellifera infected with deformed wing virus type A, and produced by Traniello et al. (2020) in Illumina HiSeq 4000 platform with Chromium Next GEM Single Cell 3สน library preparation. This RNA-seq data was 34.2G bases in length and 18.4Gb in size. Mitogenome reference was a verified RefSeq data and 16,569 bp in length with non-coding A+T- rich region.