Annotation of candidate loci and gene ontology
Of the 213 candidate loci, 209 were successfully aligned to the M. ochrogaster genome. Eight loci were located in exons, 86 in introns, and 115 in intergenic regions. We identified 74 genes associated with the candidate loci, some genes contained several loci (Table S5). Several of these genes were associated with functions in lipid metabolism, energy homeostasis, and immunity (Table 2). Among GO terms associated with the genes, six were significantly enriched in our data set, including ”regulation of respiratory burst” and ”dicarboxylic acid transport” (Table S6).
Table 2. Candidate genes detected by at least two genome scan methods, and associated with lipid metabolism, energy homeostasis and immunity. Locus refers to the location on the M. ochrogaster reference genome (Chromosome_base-position). Method of outlier detection (L: LFMM, Ba: Bayenv2, RDA: Redundancy Analysis)