Animals
BALB/c mice were purchased from Charles River (Margate, Kent, UK).
C57BL/6 and Ifnar1–/– (C57BL/6 background)20 mice were bred in-house at the University of
Edinburgh. Eight – 12 week old mice were infected by oral gavage with
200 stage 3 H. polygyrus larvae. Some animals were administered
20 µg of anti-CCR2 antibody (MC-21, gift M. Mack) by intra-peritoneal
injection seven and nine days later. Other animals were given
2x106 enriched bone marrow monocytes by intravenous
injection. Some animals were infected with RSV (105plaque forming units) intranasally ten days after H. polygyrusinfection. After monocyte transfer RSV was administered 30 minutes
later. All procedures were approved by local ethical review committee
and the UK Home Office.