L-gene real time PCR
Lungs were homogenized in 1 mL of TRIzol (ThermoFisher Scientific;
Waltham, MA, USA). cDNA was made from the two μg extracted RNA using HC
cDNA RT kit (ThermoFisher Scientific; Waltham, MA, USA) according to
manufacturer’s instructions. Custom primers and probe for RSV L gene
were used as previously described6.