Colony forming assays
Bone marrow colony forming assays were performed with MethoCult GF M3434
(StemCell Technologies; Cambridge, Cambridgeshire, UK) per
manufacturer’s instructions. Bone marrow from hind limb tibia and femur
flushes was washed, 70 µM filtered and then resuspended in MethoCult GF
M3434 at 1x104 cells per 35mm culture dish in
duplicate. Plates were incubated at 37oC 5%
CO2 in humidified chambers. After 10-12 days colonies
were characterised to lineage and counted.