Colony forming assays
Bone marrow colony forming assays were performed with MethoCult GF M3434 (StemCell Technologies; Cambridge, Cambridgeshire, UK) per manufacturer’s instructions. Bone marrow from hind limb tibia and femur flushes was washed, 70 µM filtered and then resuspended in MethoCult GF M3434 at 1x104 cells per 35mm culture dish in duplicate. Plates were incubated at 37oC 5% CO2 in humidified chambers. After 10-12 days colonies were characterised to lineage and counted.