Allplex SARS-CoV-2 assay
This assay is a conventional RT-PCR assay for detecting SARS-CoV-2; it
targets the E gene, nucleocapsid (N ) gene, RNA-dependent
RNA polymerase (RdRP ) region of the orf1ab gene, and spike
(S ) gene, with the latter two detected on the same fluorescence
channel. The RT-PCR test was performed in the Hanyang University Guri
Hospital or Seegene Medical Foundation (Seoul, Korea) laboratory.
Nucleic acid extraction for Allplex SARS-CoV-2 assay was performed using
Seeprep 32 (Seegene Inc., Korea) at the Hanyang University Guri Hospital
or MagNA Pure 96 (Roche, Switzerland) at Seegene medical foundation
according to the manufacturer’s instructions. Amplification and
detection were performed on a Bio-Rad CFX96 thermocycler (BioRad
Laboratories, The Netherlands). The results were interpreted using
Seegene Viewer data analysis software. When the Ct values of all three
fluorescence channels (E , N , and RdRP /Sgenes) were within the cutoff of 40.0 cycle, the result was considered
positive.4