Statistical analysis
All statistical analyses were performed using MedCalc (version 20.027; MedCalc, Ostend, Belgium). Overall agreement, positive percent agreement (PPA), and negative percent agreement (NPA) of the STANDARD M10 were calculated based on the results of the Allplex assay. The agreement level was determined based on Cohen’s kappa coefficient values. Inconclusive results were considered negative results. Ct values of the two assays were analyzed using Wilcoxon signed rank test. All tests were two sided, and results with P <0.05 were considered significant.