4.7 Interference Study
4.7.1 Interference due to trimethylamine : Three different
reactions were set up with the spiked concentration of TMAO. The
concentrations ranged from 20 µM to 80 µM of TMAO while keeping the
trimethylamine constant at 50 µM. The high concentration of
trimethylamine was chosen because it is the substrate of our enzyme. The
controls did not have IACN to remove the trimethylamine.
4.7.2 Metal ion: The method developed was checked for metal ion
interference; 50 µM of metal ion solutions were used in the reaction
mixture with one mM TMAO. As mentioned in section 2.5, the reaction was
performed and studied for metal ion interference.