Results and
discussions
2.1 TMAO Assay
Optimization
Figure 1 shows that the absorbance at 370
nm and 652 nm decreases significantly at pH 1.0. The method is
susceptible to the change in pH of the solution. The change in the
proton concentration affects the amount of TMAO being detected. Thus,
determining a pH range where TMAO detection is quantitative is
imperative. A pH range where the change in absorbance is more sensitive
is a better choice for detection because that will ensure a high
sensitivity of the method. Therefore, a pH range of 2.0 to 2.5 was
chosen for TMAO detection.