Figure 3 : Interference in the TMAO quantification due to the presence of trimethylamine.
Metal ions
Different metal ions usually found in biological systems often interfere with TMA assays; hence we checked if these metal ions were potent interfering agents causing any significant changes in our assay. As shown in Table 1, no interference is demonstrated by Mg and Mn ions. Except for Co, all the other metal ions showed mild deviation during the assay with a percentage error well within 5%.
Table 1: Interference of different metal ions in TMAO detection