Comparing telomere length assayed using WGS and qPCR
To quantify the relationship between telomere length calculated using qPCR and WGS data we used a Pearson correlation. First, relative telomere length measured by qPCR was correlated with mean telomere length estimates calculated using Computel, K-seek, and TRIP. Then, to evaluate the role of genome coverage to estimates of telomere length we correlated telomere length measured by qPCR with re-assessed telomere length accounting for genome coverage (See Equation 2) from K-seek and TRIP.