Statistical analysis of relation between Cancer manifestation and this disease causing factors
The protein density and mRNA density (in surrounding and tumor) from western blot and RT-PCR data suggests that both PD and MD of 1E1 and HIFα are significantly increased in the tumor groups (Table 1 and Fig 1). Present correlation study suggests that tissue MDA level is positively associated with SULT1E1 tissue expression. The 1E1 is an adaptive enzyme that catalyze the inactivation of E2 by forming E2S. More oxidative stress as evident in high MDA level has some initial role in active E2 nullification. MDA is negatively associated with HIFα expression. At the initial phase of MDA related stress response has more adaptive responses of lower HIFα. But in breast cancer tissue it is noticed that, at higher disease state with prolonged oxidative stress exposure and high MDA level HIFα is increased significantly and Nrf2 is shown to positively associated with HIFα induction. NPSH and uric acids are regarded as the endogenous soluble antioxidants those may have some protective role against the oxidant-stress-induced 1E1 regulations and Nrf2, HIFα induction. Present results and correlation data suggest that NPSH and uric acids are negatively correlated with HIFα expression. Lower antioxidant level augments the HIFα level (Table-2). The data from this table are represented from DAS and chalcone related studies shown in the figure from in fig 4, 5 and 6. Table 3 suggests that in control, DAS and Chalcone groups, NPSH and 1E1 are found to be associated. When in control and in DAS these are positively associated in Chal- group NPSH and 1E1 are shown to be negatively associated. No significant association was noticed in DAS and Chalcone groups.
Table 4 suggests that, in case of time-based exposure NPSH and 1E1 and 1E1 are found to be positively associated and 3 and 24 hrs of exposure. No significant relation was noticed in 5 hrs exposure. New sample size and internal variability may be the cause for this.