In vivo experiment
Following a single dosage of DAS on day 1, the hepatic tissue animal group treated with DAS demonstrated a progressive increase in the SULT1E1 expression with increasing duration. SULT1E1 expression was comparable to DAS 48 hours after DAS 2nd dose group. On the other hand, in 24 and 48 hours groups of chalcone treated animals expressed highest SULT1E1 but the expression gradually decreased with increasing duration. The combination group (DAS + Chal) showed an expression level less than the DAS or Chalcone alone group but greater than the control group (Fig-4a).