Oxidative stress related parameters; Malondialdehyde (MDA) and Non protein soluble thiol (NPSH)
The level of non protein thiol in vitro experiment increased after 3, 5 and 24 hrs of incubation with DAS. Das+ Chalcone incubation also causes NPSH induction as compared to the control cells (Fig-5a). In the in vivo experiment, NPSH was negligibly increased in the DAS alone and Chalcone alone treatedgroup, whereas a slight increase was noticed in DAS+Chal, 48 hr, 72hr and 2nd dose treatment (Fig-5b). Serum NPSH was found to be reduced in the Chalcone alone group, and the other entire group had NPSH similar to the control, showing no changes in the redox status in terms of NPSH.
Elevated MDA was seen in DAS-treated mice, demonstrating high MDA production with the second dose of DAS, suggesting that DAS does appear to trigger lipid peroxidation. During 48 and 72 hours, Chalcone 2nd dosage and DAS + Chalcone also had a higher MDA, in contrast to the other groups, which displayed MDA similar to the control group (Fig-5c). Serum MDA levels remain stable (Fig-5g). This means that neither DAS nor Chalcone cause systemic lipid peroxidation.