Estimation of Non Protein Soluble Thiol (NPSH)
The NPSH in serum and liver tissue homogenates (prepared in 0.1 M
phosphate buffer, pH 7.4) were determined by the standard DTNB (5, 5’-
dithiobis-2-nitrobenzoic acid) method with a slight modification (26).
In brief, the protein was precipitated by trichloroacetic acid and clear
cytosol was added to 0.1 M sodium phosphate buffer containing 5 µM DTNB.
The level of NPSH was determined against a GSH standard curve.