In –vivo (24, 48, 72 hours treatment)
Animals were divided in 3 groups, Group 1-control, Group 2-Diallysulfide (DAS), 80 mg DAS/100gm b.w., Group 3- Chalcone, 4 mg/100gm b.w., Group 4- Combination of DAS (80 mg) and Chalcone (4mg). The animals were treated with the drug or drug combination by gavages. DAS and chalcone were solubilized in 0.1% ethanol (Ethanol+water) to get the required concentration. The current dose of DAS and chalcone was screened after consultation with some previously published reports (17-19). In addition, some dose response studies were performed in our lab to validate the selected dose. The present dose demonstrated significant responses on some genes expression like SULT1E1 and this dose did not initiate any toxicity which was evaluated from animal body weight, organ weight/body weight ratios, liver and kidney function test and haematology or other toxicity studies. Control group was given same amount of 0.1 % ethanol solution as. Different groups had 3-5 animals per group and animals were serially sacrificed at 24, 48, 72 hours after drug administration, a second dose was given to a single animal at 72 hour and was sacrificed on the 5th day of the experiment i.e. at 120 hours or after 48 hours of the second dose.