In - vitro Treatment for 3, 5 and 12 hours
The enzymatic activity of catalase was low in DAS, Chalcone and DAS + Chalcone combination group in comparison to the untreated tissue. Catalase Activity gradually decreased in the in the DAS treated tissues with time, and the same was true for the Chalcone treated tissue. Among all the treated groups the highest activity was found in the DAS+Chalcone treated tissue for 3 and 5 hours, however no activity was found in the DAS + Chalcone- 24 hr treated tissue (Fig-3b).