Breast tumor sample collection
The study was conducted in Oriental Institute of Science and technology
and a total of 23 breast tumor samples were obtained from the local
District Medical College and Hospital with proper ethical clearance.
Breast tumors are diagnosed clinically; breast cancers were classified
on the basis of TNM (The extent of the tumor (T), the extent of spread
to the lymph nodes (N), and the presence of metastasis (M)) staging and
grades. In some cases down staging of cancer with chemotherapy was done
prior to surgery and samples were collected. In this regard, this is to
mention that tumor sample and corresponding surrounding tissues were
collected separately soon after surgery and stored at -20°C. A small
part of the tissue was also stored in formalin for histology and
immunohistochemistry. We sincerely thank Dr. Guangping Chen of
Department of Physiological Sciences of Oklahoma State University for
providing the primary antibody against rSULT1E1 and hSULT1E1. Anti-
Nrf-2 and NFkβ antibodies were used in the current study.